Epidemiology of Respiratory Viruses Detected by Multiplex Real-time PCR in Hospitalized Patients with Lower Respiratory Tract Infections in Vietnam from 2020 to 2024

Đào tạo & nghiên cứu

Epidemiology of Respiratory Viruses Detected by Multiplex Real-time PCR in Hospitalized Patients with Lower Respiratory Tract Infections in Vietnam from 2020 to 2024

15 Tháng 06, 2026

3 Views

Epidemiology of Respiratory Viruses Detected by Multiplex Real-time PCR in Hospitalized
Patients with Lower Respiratory Tract Infections in Vietnam from 2020 to 2024

Huong Thien Pham^1,2,3*, Van Hung Pham^1,3, Nhu Hong Thi Tran^1,3, Duy Khanh Tran^1,3,
Thao Hanh Thi Nguyen ^1,3, Quang Duy Ha^1,3, Anh Hong Pham^1,2,4

¹Vietnam Research and Development Institute of Clinical Microbiology, Ho Chi Minh city 70000, Vietnam;

²Pham Ngoc Thach University of Medicine, Ho Chi Minh city 70000, Vietnam; ³NK-Biotek Co.

BACKGROUND

Respiratory tract infections (RTIs) remain a leading cause of global hospitalization and mortality. The COVID-19 pandemic significantly disrupted traditional viral circulation and seasonal patterns. Large-scale surveillance using advanced techniques, such as multiplex real-time PCR (MLP-rPCR), is essential for preventive strategies and antimicrobial stewardship.

AIMS OF THE STUDY

This study aimed to assess viral detection trends in hospitalized RTI cases in Vietnam from 2020 to 2024 and highlight the necessity of comprehensive molecular screening.

MATERIAL AND METHODS

We conducted a five-year retrospective analysis of 15,936 respiratory clinical samples from hospitalized patients. The workflow of viral detection using an in-house MLP-rPCR assay targeting 17 respiratory viruses is shown below.

CONCLUSIONS

This study demonstrates a significant shift in the respiratory viral circulation in Vietnam following the COVID-19 pandemic. MLP-rPCR is a practical and reliable tool for resource-limited settings. Routine molecular testing can enhance diagnostic accuracy, guide clinical management, and strengthen public health surveillance.

REFERENCES

Ly, V.K.; Pham, V.H.; Van Ly, X.; Pham, P.M. Bacterial and viral co-infections in community-acquired pneumonia in adults: a prospective study of multiple hospital centers. MedPharmRes 2024, 8, 153–161. https://doi.org/10.32895/UMP.MPR.8.3.17.
Tang, Z.; Fan, H.; Tian, Y.; Lv, Q. Epidemiological characteristics of six common respiratory pathogen infections in children. Microbiol. Spectr. 2025, 13, e0007925. https://doi.org/10.1128/spectrum.00079-25.
Burk, M.; El-Kersh, K.; Saad, M.; Wiemken, T.; Ramirez, J.; Cavallazzi, R. Viral infection in community-acquired pneumonia: a systematic review and meta-analysis. Eur. Respir. Rev. 2016, 25, 178–188.

Đào tạo & nghiên cứu

Epidemiology of Respiratory Viruses Detected by Multiplex Real-time PCR in Hospitalized Patients with Lower Respiratory Tract Infections in Vietnam from 2020 to 2024

Tin liên quan

Liên kết